OK, this will reproduce the issue.

Create an empty project, then a form (Form1), then drop an UltraGauge on it.

In the dialog that opens when the UltraGauge is placed on the form, select the BasicClock preset. That will create three scales with one needle each.

Open the code behind the form and add this;

Imports Infragistics.UltraGauge.Resources

Public Class Form1

    Private Sub Form1_Load(ByVal sender As Object, ByVal e As System.EventArgs) Handles Me.Load

        ' this code works fine

        Dim RadialGauge1 As RadialGauge = UltraGauge1.Gauges(0)
        Dim RadialScale1 As RadialGaugeScale = RadialGauge1.Scales(0)
        Dim RadialGaugeNeedle1 As RadialGaugeNeedle = RadialScale1.Markers(0)

        Dim RadialScale2 As RadialGaugeScale = RadialGauge1.Scales(1)
        Dim RadialGaugeNeedle2 As RadialGaugeNeedle = RadialScale2.Markers(0)

        Dim RadialScale3 As RadialGaugeScale = RadialGauge1.Scales(2)
        Dim RadialGaugeNeedle3 As RadialGaugeNeedle = RadialScale3.Markers(0)

        ' this code does not

        'Dim RadialGaugeNeedle4 As RadialGaugeNeedle = UltraGauge1.Gauges(0).Scales(0).Markers(0)

        RadialGaugeNeedle1.Value = 3.5
        RadialGaugeNeedle2.Value = 30
        RadialGaugeNeedle3.Value = 37

    End Sub

End Class

Now try the form. Clock will display 03:30:37.

Next, comment out all the code between ' this code works fine and 'this code does not, and uncomment the line after 'this code does not.

Error appears saying

'Scales' is not a member of 'Infragistics.UltraGauge.Resources.Gauge'

Reminder that I said this is probably me overlooking something obvious.

Here is the sample   

Hello ellswrth,

Thanks for provided code. I was able to reproduce your issue. You just missed to cast your object to RadialGauge. For example:

RadialGaugeNeedle RadialGaugeNeedle4 = ((RadialGauge)ultraGauge1.Gauges[0]).Scales[0].Markers[0] as RadialGaugeNeedle;

Please take a look at the attached sample and video file for more details.

Regards